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In 2260, Sebastian was summoned by Kosh to Babylon 5, where he was charged with interrogating Ambassador Delenn. Sebastian focused his interrogation around the Vorlon's question, "Who are you?" It's unknown what became of Mr. Sebastian following his assignment to Babylon 5, especially given the later departure of the First Ones from the galaxy, though Sebastian himself expressed that perhaps the Vorlons would finally allow him to die.
While it is inferred that there are other Inquisitors, and that Sebastian has been deployed from time to time over the centuries, Sebastian is the only known Inquisitor.